Wilson, Geoffrey G. and Wang, Hua and Heiter, Daniel F. and Lunnen, Keith D. (2012) Restriction Enzymes in Microbiology, Biotechnology and Biochemistry. Encuentro: Revista Académica de la Universidad Centroamericana (93). pp. 19-48. ISSN 0424-9674
|
Text
encuentro93-1.pdf - Published Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (1MB) | Preview |
Abstract
Since their discovery in the nineteen-seventies, a collection of simple enzymes termed Type II restriction endonucleases, made by microbes to ward off viral infections, have transformed molecular biology, spawned the multi-billion dollar Biotechnology industry, and yielded fundamental insights into the biochemistry of life, health and disease. In this article we describe how these enzymes were discovered, and we review their properties, organizations and genetics. We summarize current ideas about the mechanism underlying their remarkable ability to recognize and bind to specific base pair sequences in DNA, and we discuss why these ideas might not be correct. We conclude by proposing an alternative explanation for sequence-recognition that resolves certain inconsistencies and provides, in our view, a more satisfactory account of the mechanism.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Biotecnología, Microbiología, Biología molecular, Enzimas, Metabolismo |
| Subjects: | 500 Ciencias Naturales y Matemáticas > 570 Ciencias de la vida, Biología > 576 Genética y evolución |
| Divisions: | Dependencias Académicas > Vicerrectoria Académica > Dirección de Investigación y Proyección Social |
| Depositing User: | Rogerio Medina |
| Date Deposited: | 04 Nov 2014 14:23 |
| Last Modified: | 28 Sep 2015 18:02 |
| URI: | http://repositorio.uca.edu.ni/id/eprint/1389 |
Actions (login required)
 |
View Item |
